Jul
26
A Step Closer To Enzymes Producing Hydrogen
July 26, 2017 | Leave a Comment
Ruhr-University Bochum researchers have clarified the crucial catalytic step in the production of hydrogen by enzymes. The enzymes, called [FeFe]-hydrogenases, efficiently turn electrons and protons into hydrogen. Therefore they are candidates for the biotechnological production of the potential energy source.
Professor Thomas Happe, one of the authors of the study said, “In order to produce hydrogen on an industrial scale with the aid of enzymes, we must precisely understand how they work.”
The electron-transfer path is shown in yellow, the PTP is depicted in blue. H2 is released from, or reaches the 2FeH cluster through hydrophobic gas channels (green). At the catalytically pivotal 2FeH site, the presumptive first step in the catalysis of H2 oxidation (H2 heterolysis) is depicted, resulting in the unequal intermediates H+, binding at the adt-bridge (Lewis base) and H−, binding at Fed (Lewis acid). Image Credit: Ruhr-University Bochum. Click image for the largest view.
Hydrogenases can work in two directions: they turn protons and electrons into hydrogen, and also split hydrogen into protons and electrons. These reactions take place at the active center of the hydrogenase, which is a complex structure comprising six iron and six sulphur atoms, called the H-cluster. During the catalytic process, this cluster passes through numerous intermediate states.
When molecular hydrogen (H2) is split, the hydrogen molecule initially bonds to the H-cluster.
Dr. Winkler explained, “Hydrogenase researchers were always convinced that H2 had to split unevenly in the first step of the reaction.” The idea: A positively charged proton (H+) and a negatively charged hydride ion (H-) are created, which then continue to react quickly to form two protons and two electrons. “The hydride state of the active enzyme, in which the hydride ion is thus bonded to the active center, is highly unstable – so far no one has been able to verify this,” said Winkler. This is precisely what the researchers have now achieved.
Using a trick, they augmented the H-cluster state with the hydride ion, so that it could be verified spectroscopically. When hydrogen is split, a chemical equilibrium is achieved between the reaction partners involved – protons, hydride ions and hydrogen molecules. The concentrations of the three hydrogen states are determined by a dynamic equilibrium of catalytic H-cluster states. When the researchers added large quantities of protons and hydrogen to the mixture from outside, they tipped the balance – in favor of the hydride state. The active center with the negatively charged hydride ion accumulated in a larger quantity; enough to be measurable.
The team also demonstrated the hydride intermediate state, which also occurs during hydrogen production, in further experiments with hydrogenases that had been altered in a specific manner.
Professor Happe summarized, “We were thus able to demonstrate the catalytic principle of these hydrogenases in an experiment for the first time. This provides a crucial basis for reproducing the highly effective catalytic mechanism of the H-cluster for the industrial production of hydrogen.” The enzymes can convert up to 10,000 hydrogen molecules per second.
Is it a breakthrough? Looks very good. The idea of using hydrogen alone as a fuel has immense potential. Somehow somewhere, maybe this team’s work will get us there. But the storage conundrum is still out there. But the curious notion in this is, it might be a hydrogen producer on demand rather than a hydrogen producer needing large storage for later use.
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