Jul
18
Serious Genetic Engineering Begins for Jatropha
July 18, 2013 | Leave a Comment
John E. Carlson, professor of molecular genetics at Penn State has begun the research into genetically engineering the jatropha plant for drought resistance. Jatropha is a high potential biofuel plant grown in marginal land areas where droughts are common. Carlson leads an international group of scientists that has identified the first step toward engineering a hardier jatropha variety.
Jatropha Plantation Single Plant. Click image for the largest view.
Jatropha has seeds with high oil content. But the oil’s potential as a biofuel is limited because, for large-scale production, this shrub-like plant needs the same amount of care and resources as crop plants.
Carlson said, “It is thought that jatropha’s future lies in further improvement of jatropha for large-scale production on marginal, non-food croplands through breeding and/or biotechnology. The more that is known about the genetic basis of jatropha’s key attributes such as drought tolerance, the more readily jatropha improvement will progress.”
Jatropha Commercial Efforts Map. Click image for the largest view.
According to Carlson, Jatropha currently grows best in tropical countries and is already being cultivated as a biofuel on a small scale in India, Southeast Asia and Africa. Breeding a strain that could do well in arid, barren conditions could enable mass cultivation, but large-scale production may still be decades away.
Researchers looked at a little known gene – JcPIP1 – because a similar gene in the model plant Arabidopsis is known to play a role in drought response. They also examined JcPIP2, a potential drought response gene in Jatropha identified in 2007 by researchers at Sichuan University.
The research paper about the findings has been published in the Journal of Plant Physiology.
The fundamentals are worth noting. The JcPIP genes code for membrane channels called aquaporins, which are responsible for transporting and balancing water throughout the plant, though exactly how each gene affects aquaporin behavior under environmental stress remains unclear.
However, researchers have found that JcPIP1 and JcPIP2 are expressed at different times during a stressful situation, which hints at what roles they play in response and recovery.
By growing unmodified Jatropha samples in conditions simulating high soil salinity and low water availability, the researchers showed that Jatropha was normally more vulnerable and slower to recover from high salinity than from drought conditions.
Using a tobacco mosaic virus to transiently transform Jatropha, the researchers created plants in which JcPIP2 or JcPIP1 was temporarily disabled. They subjected the modified samples to six days of stress and six days of recovery. To gauge the plants’ stress responses, they noted physical changes and measured root damage, leaf growth, electrolyte leakage in the leaves, and sap flow and volume.
The researchers found that these stress responses were about the same between the two variants under drought conditions. However, plants with JcPIP1 disabled were slower to recover from salt damage.
Analysis of plant parts during the stress and recovery stages showed that JcPIP2 was mostly active in the early stages of stress while JcPIP1 expression was greater during recovery. The timing indicates that JcPIP1 may be crucial in helping Jatropha recover from damage while JcPIP2 may play a role in damage prevention.
How the two genes affect other plant functions remains unknown, and how large a part they play in the entire network of drought resistance relies on further study.
Carlson explains, “Plants have complex genetic and biochemical pathways for environmental stress resistance, that includes (multiple) genes and pathways. This inherent redundancy in stress responses ensures survival under varying environmental conditions, and provides many possible approaches to improving resistance.”
According to the research team, the next step is to find how the JcPIP genes work at the cellular level, which can provide more detailed profiles of each gene’s exact function.
Other researchers on the international project include lead investigator Sung Ju Ahn and Ha-Young Jangat, Chonnam National University, Korea; Seong-Wook Yang, associate professor of plant biology and biotechnology,University of Copenhagen; and Yang-Gyu Ku, Wonkwang University, Korea.
It’s getting serious for jatropha now. Jatropha was a little known, obscure plant of little consequence only a few years ago. Now the work is underway to build up the genetic knowledge to modify the plant on the genetic engineering fast track. That’s good news for perhaps millions of subsistence farmers and fuel starved third world countries.
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