Schematic representation of the D.desulfuricans (top, PDB code 1HFE) and C. acetobutylicum (bottom) hydrogenases orientation on a graphite electrode modified with 4-nitrobenzendiazonium salt and 4-carboxyphenyl
diazonium salt respectively. In red are negatively charged areas, in blue positively charged areas are shown. The surface vacuum electrostatic charges map was generated using Pymol v 0.98. At the moment there is no crystal structure from CaHydA, the crystal structure from  CpI (PDB code 1FEH) was used as a model based on the sequence similarity between both proteins.

Image Credit: Wiley

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